TABLE 1.
Purification of 1,2,4-trihydroxybenzene 1,2-dioxygenase from Burkholderia sp. strain AK-5
| Fractiona | Total activity (U) | Total protein (mg) | Sp act (U · mg−1) | Recovery (%) |
|---|---|---|---|---|
| 1 (cell extract) | 340 | 2,900 | 0.12 | 100 |
| 2 (streptomycin sulfate) | 240 | 2,700 | 0.09 | 71 |
| 3 (ammonium sulfate) | 230 | 1,400 | 0.16 | 68 |
| 4 (DE52) | 80 | 150 | 0.53 | 24 |
| 5 (DEAE-Cellulofine A-800) | 30 | 20 | 1.5 | 8.8 |
| 6 (Phenyl-Cellulofine) | 6.0 | 1.1 | 5.5 | 1.8 |
| 7 (Cellulofine GCL-1000 sf) | 3.0 | 0.24 | 13 | 0.9 |
a
Fractions 1 to 7 refer to the fractions obtained at the end of steps 1 to 7, respectively, of the purification procedure. See the text for details.