TABLE 1.
PCR-RFLP system of cry1I genes
| Gene or primer | Accession no. | Sequenceb | Positions | Coding region (nt)c | Product size (bp) | Fragment size (nt) (bp) with Bsp119I and BanI |
|---|---|---|---|---|---|---|
| Primers | ||||||
| S5uni | GCTGTCTACCATGATTCGCTTG | |||||
| S3uni | CAGTGCAGTAACCTTCTCTTGC | |||||
| Genes | ||||||
| cry1Ia1 | X62821 | GCTGTCTACCATGATTCGCTTG | 760-781 | 355-2514 | 1,584 | 141, 444, 571, 47, 381 |
| CAGTGCAGTAACCTTCTCTTGC | 2343-2322 | |||||
| cry1Ib1 | U07642 | GCcGTCTACCATGAaTCGCTTG | 642-663 | 237-2396 | 1,584 | 1,156, 47, 381 |
| CAGTGCAGTAACCTTCTCTTGC | 2225-2204 | |||||
| cry1lc1 | AF056933 | GCTGTCTACCATGAaTCGCTTG | 406-427 | 1-2160 | 1,584 | 522, 634, 47, 381 |
| CAGTGCAGTAACCTTCTCTTGC | 1989-1968 | |||||
| cry1Id1 | AF047579 | GCTGTCTACCATGAaTCtCTTG | 907-928 | 502-2661 | 1,584 | 141, 444, 999 |
| CAtTGCAGTAACtcTaTCTTGt | 2490-2469 | |||||
| cry1Ie1a | AF211190 | GCTGTCTACCATGAaTCGCTTG | 715-736 | 310-2469 | 1,584 | 585, 571, 47, 381 |
| CAGTGCAGTAACCTTCTCTTGC | 2298-2277 |
a
The novel gene found in this study
b
Lowercase letters indicate nucleotides that differ from those in the primer sequences.
c
nt, nucleotides.