TABLE 2.
HA of F. psychrophilum and sialidase-treated rainbow trout erythrocytesa
| Bacterial strain | Incubation time and erythrocyte type
|
|||||||
|---|---|---|---|---|---|---|---|---|
1 and 2 h
|
5 h
|
10 h
|
20 and 48 h
|
|||||
| Control | Sialidase treated | Control | Sialidase treated | Control | Sialidase treated | Control | Sialidase treated | |
| 900406-1/1 | − | − | − | − | − | − | − | − |
| 69/3A | − | − | − | − | − | − | − | − |
| 66/3A | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ |
| 43/2A | ++ | ++ | ++ | + | ++ | + | ++ | − |
| 950106-1/1 | ++ | ++ | ++ | ++ | ++ | +* | ++ | + |
| NCMB1947 | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ |
a
Time intervals depict the incubation times of erythrocytes with sialidase before testing HA. Control erythrocytes were incubated with PBS. ++, total HA; +, partial HA; −, no HA. *, 950106-1/1 caused a weak HA in one of two experiments. All other samples showed the same result in duplicate experiments.