TABLE 3.
Summary of the data obtained from the clone libraries and DGGE
| Phylotype | Sequenced clones and DGGE bandsa | Total no. of clones | Closest GenBank relative (% similarity)b | Closest GenBank cultured relative (% similarity)b | Clones with the following ITS types:
|
Clusterc | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | Unique | ||||||
| 1 | Fr397, Fr114, Fr311, Fr401, Fr025, Fr252 | 10 | Uncultured Antarctic bacterium LB3-46 (99) | Oscillatoria sp. strain OH25 (90) | Fr397, Fr311 | XII | ||||||
| 2 | Fr032, Fr288, FrF4 | 2 | Phormidium mucicola M221 (95) | Fr032 | XIV | |||||||
| 3 | Fr239 | 1 | Phormidium mucicola (95) | Fr239 | XIV | |||||||
| 4 | Fr094, Fr297, Fr246, Fr350, Fr396 | 13 | Uncultured Antarctic bacterium CSC1 (96-99) | Leptolyngbya sp. strain PCC9207 (90) | Fr297, Fr350 | Fr094 | Fr246 | XI | ||||
| 5 | Fr132, Fr044, BGC-, Fr019, FrE203, FrF5 | 4 | Uncultured bacterium WH12 (92) | Leptolyngbya sp. strain PCC9207 (91) | Fr132 | X | ||||||
| 6 | Fr285 | 1 | Uncultured bacterium WH12 (93) | Leptolyngbya sp. strain PCC9207 (91) | X | |||||||
| 7 | Fr023 | 2 | Uncultured Antarctic bacterium LB3-76 (99) | Oscillatoria amphigranulata strain 11-3 (91) | Fr121 | IX | ||||||
| Fr121 | Uncultured Antarctic bacterium CSC14 (98) | Oscillatoria amphigranulata strain 11-3 (91) | ||||||||||
| 8 | Fr304 | 1 | Nostoc commune NIVA-CYA308 (99) | Fr304 | VII | |||||||
| FrF1, FrF1bis | Nostoc sp. (Nephroma helveticum cyanobion) strain 33 (99) | |||||||||||
| 9 | BGC-Fr056, BGC-Fr023, BGC-Fr005, BGC-Fr020, BGC-Fr067, FrF3 | 6 | Geitlerinema sp. strain PCC9222 (97-99) | BGC-Fr056, BGC-Fr005, BGC-Fr067 | BGC-Fr023 | V | ||||||
| 10 | BGC-Fr032, BGC-Fr025, BGC-Fr068, BGC-Fr072, BGC-Fr078, BGC-Fr080 | 10 | Phormidium sp. strain Ant-Orange (99) | BGC-Fr068 | BGC-Fr032, BGC-Fr080 | Fr048 | I | |||||
| Fr048 | Lyngbya sp. strain UTCC296 (99) | |||||||||||
| 11 | BGC-Fr054, BGC-Fr006, BGC-Fr030, BGC-Fr044, BGC-Fr060, BGC-Fr073 | 24 | Uncultured Antarctic bacterium LB3-1 (97-98) | LPP group Antarctic cyanobacterium QSSC8cya (97-98) | BGC-Fr054 | BGC-Fr060 | XXI | |||||
| 12 | Fr147 | 1 | Uncultured soil crust cyanobacterium lichen 4 (96) | Phormidium ambiguum M71 (92) | Fr147 | III | ||||||
| 13 | Fr005 | 1 | Uncultured soil crust cyanobacterium lichen 4 (96) | Phormidium ambiguum M71 (92) | Fr005 | III | ||||||
| 14 | FrE313 | 1 | Pseudanabaena sp. strain PCC6903 (98%) | XIII | ||||||||
| 15 | Fr127 | 1 | Uncultured Antarctic bacterium CSC17 (94) | Phormidium mucicola M221 (90) | Fr127 | IX | ||||||
a
The 16S rRNA genes of the following clones have been completely sequenced: Fr397, Fr032, Fr239, Fr094, Fr297, Fr132, Fr121, Fr304, BGC-Fr056, BGC-Fr032, Fr048, BGC-Fr054, Fr147, Fr005, FrE313, and Fr127. The following clones are duplicate clones according to their ARDRA patterns: Fr025, Fr252, Fr396, BGC-Fr005, BGC-Fr020, BGC-Fr067, BGC-Fr078, BGC-Fr080, and BGC-Fr073. Clones Fr019 and Fr285 were detected as chimeras in the complete 16S rRNA gene sequence. FrF4, FrF5, FrF1, FrF1bis, and FrF3, are DGGE bands.
b
Levels of similarity were determined by BLAST.
c
See Fig. 2.