TABLE 2.
Characterization of products formed from isoflavonoids catalyzed by the BphA and BphB of Burkholderia sp. strain LB400
| Substratea | Products
|
||||
|---|---|---|---|---|---|
| No. | tr (min) | Yieldb (%) | Prominent ionsc (m/z) | Ring B | |
| 7-OH-8-CH3-ISF (1a) | 1 | 27.3 | 2-4 | 412 (M+, 9), 397 (100), 73 (64) | 2′-OHd |
| 2 | 29.5 | 96-98 | 500 (M+, 9), 485 (79), 73 (100) | 2′,3′-Di-OHe | |
| 7-OH-ISF (2a) | 1 | 26.5 | 10-15 | 398 (M+, 9), 383 (100), 73 (80) | 3′-OHd |
| 2 | 27.8 | 15-20 | 488 (M+, 10), 473 (7), 397 (46), 383 (76), 73 (100) | 3′,4′-Dihydrodiolf | |
| 3 | 28.4 | 10-15 | 398 (M+, 93), 383 (92), 73 (100) | 4′-OHd | |
| 4 | 28.6 | 55-60 | 486 (M+, 9), 471 (100), 73 (60) | 3′,4′-Di-OHg | |
a
ISF, isoflavone.
b
Deduced from TIC peak areas.
c
The mass ion is underlined. The relative abundance(s) (expressed as the percent base peak) of each ion is given in parentheses.
d
Assignment is based on tr values and on the assumption that this is a dehydration product.
e
Identified by comparison of the mass spectrum and by chromatographic coelution with the defluorinated product of isoflavone 1c.
f
Assignment is based on the identification of the follow-up product generated by BphA and BphB.
g
Identified by NMR analysis.