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Diagram of the construction of the ΔΨ packaging plasmid. To delete the major packaging signal of HIV, we have subcloned the LTR (plasmid pLTR) and the gag gene (plasmid pGAG), leaving a 37-base gap between these two fragments. These two fragments have been ligated together (plasmid pLTR/GAG) and with the rest of the HIV genome (plasmid ΔΨ).
