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. 1996 Nov 26;93(24):14070–14075. doi: 10.1073/pnas.93.24.14070

Figure 5.

Figure 5

Lack of infectivity of the Ψ422 and g cell line supernatants. (A) Quadruplicate cultures of Molt4/8 cells were exposed to various amounts (0.001 to 1000 pg of p24 antigen) of wild-type HIV-1-MN (♦) or mutant (Ψ422, ▪; gV, ○; gZ, ▵) virions. At day 28 cells were tested for HIV-1 infection by p24 capture assay. The percentage of cultures which were HIV p24-positive is represented for each viral input. (B) In parallel, cells exposed to 0.1 pg of HIV-1-MN (lane 1) or to 1000 pg of Ψ422 (lane 2), gV (lane 3), or gZ (lane 4) virions for 28 days were lysed and submitted to PCR using gag-specific oligomers. A 114-nucleotide HIV-1 gag sequence was amplified only in cells infected with wild-type HIV-1-MN (Upper). As a positive control the β-actin gene was detected in the same cell lysates by PCR using specific oligomers (Lower). PCR was performed on noninfected Molt4/8 cells (lane 5) and on buffer (lane 6) as negative controls.