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Formamide and specificity study for PNA MB Bact0338. Flow cytometry analysis of E. coli (EC, perfect match), V. spinosum (VS, two mismatches), and P. limnophilus (PL, three mismatches) cells hybridized with 50 nM PNA MB Bact0338 in hybridization buffer containing different concentrations of formamide (0 to 70% [vol/vol]). a.u. indicates arbitrary units. Each value shown is the mean plus or minus standard deviation of the mean of three replicates.
