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Cytoplasmic proteins were absent from the surface-associated protein preparation from pH 5.2-grown cultures separated by 2-D PAGE. Lactate dehydrogenase (LDH) and pyruvate oxidase (POX) were abundant in the whole-cell extract (A) but were absent on the 2-D gels of the surface-associated proteins (B). Fructose bisphosphate aldolase (FBA) was identified in both intracellular and surface-associated fractions.
