TABLE 1.
Identity of S. oralis surface-associated proteins separated by 2-D PAGE
| Functional category | Spot no.a | Putative functionb | Gene symbolc | Acc. no.d | Observed migratione
|
Theoretical migrationf
|
No. of peptides matchedg | Coverageh | Ratio mean spot volume 5.2/7.0i | ||
|---|---|---|---|---|---|---|---|---|---|---|---|
| pI | Mr | pI | Mr | ||||||||
| Cellular processes and stress response | 20 | Superoxide dismutase (manganese dependent) | sodA | SP0766 | 5.1 | 24.9 | 4.9 | 22.4 | 4 | 26 | 2.23j |
| 25 | Thioredoxink | trx | SP1776 | 4.8 | 11.0 | 4.5 | 11.4 | 6 | 71 | NDEl | |
| Central and intermediary metabolism | 2 | Phosphoglucomutase | pgm | SP1498 | 4.7 | 64.5 | 4.7 | 62.7 | 7 | 17 | NDE |
| 4 | Dipeptidase | pepV | SP0623 | 4.9 | 50.7 | 4.8 | 50.8 | 6 | 12 | 2.02j | |
| 7 | Phosphoglycerate kinase | pgk | SP0499 | 5.1 | 47.0 | 4.9 | 41.9 | 9 | 24 | NDE | |
| 6 | Enolase | eno | SP1128 | 4.8 | 46.6 | 4.7 | 47.1 | 8 | 22 | NDE | |
| 8 | Glyceraldehyde-3-phosphate dehydrogenase | gap | SP2012 | 5.6 | 40.5 | 5.3 | 35.9 | 10 | 40 | 0.45j | |
| 10 | Manganese-dependent inorganic pyrophosphatase | ppaC | SP1534 | 4.7 | 35.0 | 4.6 | 33.5 | 6 | 26 | 2.67j | |
| 12 | Fructose bisphosphate aldolase | fba | SP0605 | 5.4 | 30.4 | 5.2 | 31.4 | 9 | 33 | NDE | |
| 19 | Triosephosphate isomerase | tpi | SP1574 | 5.0 | 25.8 | 4.8 | 26.5 | 5 | 27 | NDE | |
| Transport and binding | 21 | Phosphocarrier protein HPrk | ptsH | SP1177 | 4.9 | 17.5 | 4.5 | 8.9 | 4 | 41 | NDE |
| Purines, pyrimidines, nucleosides, and nucleotides | 15 | Adenylate kinase | adk | SP0231 | 5.2 | 27.4 | 5.0 | 23.7 | 8 | 30 | NDE |
| Transcription and translation | 1 | EF-G | fusA | SP0273 | 5.0 | 68.7 | 4.9 | 76.8 | 21 | 37 | 0.38j |
| 9 | EF-Tsk | tsf | SP2214 | 4.9 | 39.1 | 4.6 | 37.4 | 12 | 37 | 0.27j | |
| 17 | EF-Pk | efp | SP0435 | 4.9 | 24.5 | 4.6 | 20.6 | 8 | 54 | 0.33 | |
| 5 | EF-Tuk | tuf | SP1489 | 4.9 | 46.0 | 4.9 | 44.0 | 13 | 33 | 0.31 | |
| 16 | Ribosome recycling factor | frr | SP0945 | 6.3 | 23.7 | 6.0 | 20.7 | 6 | 30 | NDE | |
| 18 | Transcription elongation factor GreA | greA | SP1517 | 4.6 | 21.5 | 4.5 | 17.6 | 4 | 32 | NDE | |
| 24 | Ribosomal protein S6k | rpsF | SP1541 | 5.3 | 15.9 | 4.9 | 11.2 | 6 | 73 | NDE | |
| 27 | Ribosomal protein L7/L12k | rplL | SP1354 | 4.5 | 12.0 | 4.1 | 12.4 | 4 | 37 | NDE | |
| Cell envelope | 11 | Lipoprotein | SP0845 | 4.9 | 36.0 | 5.4 | 36.7 | 5 | 17 | NDE | |
| Other | 14 | Serine/threonine protein phosphatasek | SP1201 | 4.7 | 27.2 | 4.3 | 27.1 | 4 | 29 | 2.74j | |
| 23 | Bacterocin transport accessory proteink | bta | SP1499 | 5.7 | 17.2 | 5.7 | 12.9 | 4 | 42 | NDE | |
| Unknown | 3 | Conserved hypothetical protein | SP0868 | 4.2 | 60.5 | 4.8 | 46.3 | 4 | 10 | 3.26 | |
| 13 | Conserved hypothetical protein | SP1922 | 4.7 | 33.6 | 4.5 | 25.8 | 6 | 24 | 1.63 | ||
| 22 | Conserved hypothetical proteink | SP2202 | 5.8 | 15.9 | 5.2 | 10.2 | 3 | 48 | NDE | ||
| 26 | Conserved hypothetical proteink | SP0122 | 4.6 | 10.0 | 4.3 | 9.3 | 2 | 48 | NDE | ||
Spot number refers to the proteins labeled in Fig. 1.
Putative functions were assigned from the TIGR database for S. pneumoniae.
Gene symbol in the TIGR database for S. pneumoniae.
Accession number in the TIGR database for S. pneumoniae.
Calculated from data presented in Fig. 1. Mrs are in thousands.
As given in the TIGR database for S. pneumoniae.
Number of tryptic peptides observed contributing to percentage amino acid coverage.
The percentage of amino acid coverage (peptides observed/theoretical value from sequence data).
Ratio of IOD for each protein derived from cells cultured at pH 5.2 to that for the protein derived from cells cultured at pH 7.0 (mean; n = 3 for each growth condition).
Significantly different (P < 0.05) as calculated by Student's t test.
Proteins identified by LC-MS/MS. All other identifications were achieved by MALDI-TOF MS.
NDE, not differentially expressed.