TABLE 2.
Archaeal 16s rDNA and mcrA clone recovery from MOA-containing sediments and fosmid DNA librarya
| DNA | Group | Recovery (no. of clones)
|
Total no. of clones | ||||
|---|---|---|---|---|---|---|---|
| Eel River
|
Monterey Canyon F17.1 | AMIS microcosm C4.1 | Blake Ridge PC26 | ||||
| T201 | Gzfos | ||||||
| Archaeal 16s rDNA | ANME-1 | 62 | 8 | 1 | 3 | 74 | |
| ANME-2 | |||||||
| ANME-2a | 1 | 41 | 42 | ||||
| ANME-2b | 9 | 9 | |||||
| ANME-2c | 20 | 1 | 27 | 43 | 91 | ||
| Methanogen-like | 1 | 1 | 22 | 24 | |||
| Other | 6 | 49 | 31 | 5 | 91 | ||
| mcrA group | a | 13 | 4 | 9 | 25 | 51 | |
| b | + | 6 | + | ND | 6 | ||
| c | 3 | 4 | 3 | 14 | 5 | 29 | |
| d | 1 | 3 | 4 | ||||
| e | 3 | 3 | |||||
a
T201 environmental sequences and the Gzfos library were both derived from the same purified cell preparation of MOA from Eel River T201 sediment (see text). Environmental samples and fosmid library were screened with universal mcrA primers ME-1 and ME-2 and ANME-1 type b-specific mcrA primers AOM39 and AOM40 (see text). +, positive amplification with AOM39 and AOM40 primer set. mcrA group represents conceptual translation product. ND, not determined.