Table 1.
Unfractionated
|
CD8+
|
CD4+
|
||||
---|---|---|---|---|---|---|
Unstimulated | PHA | Unstimulated | PHA | Unstimulated | PHA | |
MIP-1α | ||||||
1 | 40 | 7,418 | 10 | 2,693 | 10 | 3,835 |
2* | 13 | 1,531 | 10 | 4,396 | 10 | 733 |
3 | 10 | 8,138 | 224 | 3,201 | 1729 | 6,580 |
MIP-1β | ||||||
1 | 838 | 19,305 | 59 | 20,975 | 46 | 20,308 |
2* | 104 | 24,928 | 123 | 28,357 | 19 | 4,094 |
3 | 10 | 29,773 | 171 | 23,759 | 1738 | 28,557 |
RANTES | ||||||
1 | 10 | 4,759 | 10 | 1,914 | 10 | 1,742 |
2* | 10 | 2,739 | 10 | 2,383 | 10 | 185 |
3 | 10 | 4,019 | 10 | 825 | 10 | 1,825 |
β-chemokine production in culture supernatants was assessed on day 5 post-stimulation.
CD4+ and CD8+ cells were obtained by positive selection using immunomagnetic beads rather than by negative selection.