TABLE 3.
HS-GC measurement of volatile compounds produced by ATF overexpression strains after 36 h of fermentation
| Compound | Concn (mg liter−1)a
|
||||||
|---|---|---|---|---|---|---|---|
| CMBS33 pATF1(CMBS33)s | CMBS33 pATF1(CMBS212)s | CMBS33 pATF2(CMBS33)s | CMBS33 pATF2(CMBS212)s | CMBS33 pLg- ATF1(CMBS33)s | CMBS33 ps | CMBS33 (wild type) | |
| Ethyl acetate | 273 (30.9) | 219 (24.8) | 28.4 (3.20) | 19.7 (2.23) | 16.7 (1.89) | 8.59 (0.97) | 8.86 |
| Isoamyl acetate | 49.2 (185) | 48.7 (183) | 6.94 (26.1) | 3.20 (12.0) | 1.22 (4.60) | 0.22 (0.80) | 0.27 |
| Ethyl hexanoate | 0.06 (1.10) | 0.05 (1.01) | 0.05 (0.90) | 0.07 (1.32) | 0.06 (1.07) | 0.07 (1.27) | 0.05 |
| Ethyl octanoate | 0.02 (0.46) | 0.07 (1.28) | 0.04 (0.79) | 0.07 (1.25) | 0.05 (0.88) | 0.03 (0.60) | 0.05 |
| Isoamyl alcohol | 138 (0.54) | 174 (0.69) | 224 (0.89) | 232 (0.92) | 204 (0.81) | 222 (0.88) | 253 |
| Isobutanol | 90.6 (1.11) | 78.1 (0.96) | 95.8 (1.18) | 74.8 (0.92) | 94.8 (1.17) | 85.7 (1.05) | 81.3 |
| Propanol | 45.7 (0.95) | 47.5 (0.98) | 47.2 (0.98) | 48.4 (1.00) | 48.4 (1.00) | 48.7 (1.01) | 48.3 |
| Acetaldehyde | 5.16 (0.85) | 6.34 (1.04) | 6.36 (1.05) | 6.43 (1.06) | 6.70 (1.10) | 5.95 (0.98) | 6.08 |
| DMS | 0.04 (1.06) | 0.04 (1.10) | 0.05 (1.34) | 0.05 (1.35) | 0.06 (1.38) | 0.07 (1.84) | 0.04 |
a
Results are the averages for six independent fermentations. Values in parentheses show the value relative to that for the wild type. Values that are significantly (α = 0.05) different from those for the wild-type strain are in bold. The standard deviations for large values (>1.5) were typically around 10% of the value and did not exceed 20% for any value. For small values (<1.5), the standard deviations were typically around 0.1, and did not exceed 0.4.