TABLE 1.

PCR primer sets and restriction enzymes used for the detection of tetracycline efflux genes

Target gene	Primersa (sequences)	Predicted amplified fragment size (bp)	Restriction enzyme	Restriction fragment sizes (bp)
tetA	tetAC-F (5′-CGCYTATATYGCCGAYATCAC-3′), tetAC-R (5′CCRAAWKCGGCWAGCGA-3′)	417	SmaI	267, 150
tetC		417	SalI	216, 201
tetB	tetBDEFHJ-F (5′-GGDATTGGBCTTATYATGCC-3′), tetBD-R (5′-ATMACKCCCTGYAATGCA-3′)	967	SphI	173, 302, 492
tetD		964	SphI	324, 640
tetG	tetGY-F (3′-TATGCRTTKATGCAGGTC-5′), tetGY-R (5′-GACRAKCCAAACCCAACC-3′)	917	EcoRI	368, 549
tetY		911	SphI	197, 714
tetE	tetBDEFHJ-F, tetEHJ-R (5′-AWDGTGGCDGGAATTTG-3′)	650	NdeII	77, 148, 425
tetH		650	NdeII	267, 383
tetJ		650	NdeII	117, 297, 236

^aF, forward; R, reverse.