TABLE 3.
PCR primer sets for determination of nucleotide sequences of tetracycline efflux genes
| Target gene | Primersa (sequences [5′-3′]) | Amplified fragment size (bp) | Reported size of tet gene (bp) |
|---|---|---|---|
| tetB | tetB-F (CTAATCTAGACATCATTAATTCC), tetB-R (TTTGAAGCTAAATCTTCTTTAT) | 1,397 | 1,206 |
| tetC | tetC-F (ATGAAATCTAACAATGCGC), tetC-R (TCAGGTCGAGGTGGCCCGG) | 1,191 | 1,191 |
| tetD | tetD-F (ATGAATAAACCCGCTGTCATCGC), tetD-R (AATGCCGTCCACCATACC) | 1,232 | 1,185 |
| tetG | tetG-F (AGTTTCAGGTGCGCAGC), tetG-R (CCAAWTCGCCATGACTMAAT) | 1,232 | 1,176 |
| tetY | tetY-F (ATGTCAAAATCACTTATAACCGC), tetY-R (TCTCCCGCCAAGATTTTA) | 1,191 | 1,176 |
a
F, forward; R, reverse. Amplified fragments included the full lengths of tet genes, and some also included up- and/or downstream regions.