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Proteinase K protection assay of 4BRA and 4BL3PAT. 125I-labeled toxin was incubated with C. quinquefasciatus BBMV for 1 h. Free and noninserted toxin was digested with proteinase K, and the reaction was stopped with Pefabloc. BBMV-protected toxin was separated by centrifugation, and the counts were measured.
