FIG. 6.

C. quinquefasciatus BBMV light-scattering assay. BBMV (0.2 mg/ml) were coinjected with different samples. All samples were in 10 mM HEPES buffer, pH 7.5. The samples were KCl (150 mM) (line 1), 4BRA (66 pmol of toxin/mg in 150 mM KCl) (line 2), 4BL3PAT (66 pmol of toxin/mg in 150 mM KCl) (line 3), and HEPES buffer alone (line 4). Light scattering was measured using a stopped-flow apparatus at a 488-nm wavelength with the temperature controlled at 20°C. The curves were normalized to begin from the same point. The increasing intensity is indicative of reswelling of the vesicles. The data shown are averages of two experiments.