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Ligand blot of biotin-labeled 4BRA (Cry4Ba) and 4BL3PAT (Mutant) to C. quinquefasciatus BBMV. Ten micrograms of BBMV proteins was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, blotted onto a polyvinylidene difluoride membrane, and probed with 5 nM biotinylated toxins. Arrows point to bands that are uniquely bound by 4BL3PAT. Lanes 1 and 3, BBMV; lanes 2 and 4, precipitated BBMV. Numbers between the panels are molecular masses in kilodaltons.
