TABLE 1.
Sequences of primers used in site-directed mutagenesis
| Primera | Sequence (5′→3′) | Mutant |
|---|---|---|
| Fw4BR203A | GGTCTTTAGCAGCTAGTGCTGGTGACC | 4BRA |
| Re4BR203A | GGTCACCAGCACTAGCTGCTAAAGACC | |
| Fw4BL1QTT | ACCAATACTCAAACTACAGATTTAAGATTTTTATC | 4BL1QTT |
| Re4BL1QTT | TCTTAAATCTGAAGTTTGAGTATTGGTCCAGAAATC | |
| Fw4BL2NDY | CTAATCGAGTTAATGATTATACAAAAATGGATTTC | 4BL2NDY |
| Re4BL2NDY | CATTTTTGTATAATCATTAACTCGATTAGAGGGTATTC | |
| Fw4BL3PAT | GATGTTATACCTGCGACTTATAACAGTAACAGGGTTTC | 4BL3PAT |
| Re4BL3PAT | CTGTTATAAGTCGCAGGTATAACATCAGTTTTTATATAG | |
| Fw4BL3AAT | GATGTTATAGCTGCGACTTATAACAGTAACAGGGTTTC | 4BL3AAT |
| Re4BL3AAT | CTGTTATAAGTCGCAGCTATAACATCAGTTTTTATATAG | |
| Fw4BL3GAT | GATGTTATAGGTGCGACTTATAACAGTAACAGGGTTTC | 4BL3GAT |
| Re4BL3GAT | CTGTTATAAGTCGCACCTATAACATCAGTTTTTATATAG | |
| Fw4BL3GAV | GATGTTATAGGTGCGGTTTATAACAGTAACAGGGTTTC | 4BL3GAV |
| Re4BL3GAV | CTGTTATAAAGCGCACCTATAACATCAGTTTTTATATAG | |
| Fw4BL3PAA | GATGTTATACCTGCGGCTTATAACAGTAACAGGGTTTC | 4BL3PAA |
| Re4BL3PAA | CTGTTATAAGTCGCAGGTATAACATCAGTTTTTATATAG | |
| Fw4BL3AAA | GATGTTATAGCTGCGGCTTATAACAGTAACAGGGTTTC | 4BL3AAA |
| Re4BL3AAA | CTGTTATAAGCCGCAGCTATAACATCAGTTTTTATATAG |
a
The sets of complementary primers for creating the mutants are grouped together.