TABLE 2.
Bioassay results for four species of mosquitoes
| Toxin | LC50 (ng/ml)a
|
|||
|---|---|---|---|---|
| A. quadrimaculatus | A. aegypti | C. quinquefasciatus | C. pipiens | |
| Cry4Aa | ND | 600 (1,300-2,000)b | 980 (680-1,490)c | 400 (300-500)b |
| Cry4Ba | 25 (18-32) | 61 (28-175) | >80,000d | >20,000g |
| 4BwtGAV | 745 (607-962) | 174 (117-280) | >20,000f | ND |
| 4BRA | 21 (15-29) | 21 (5-51) | >80,000e | >20,000d |
| 4BL1QTT | >20,000d | >20,000c | >20,000d | ND |
| 4BL2NDY | >20,000d | >20,000d | >20,000d | ND |
| 4BL3PAT | 44 (40-50) | 53 (19-91) | 365 (267-529) | 95 (69-130) |
| 4BL3AAT | 16 (8-23) | 68 (19-140) | 1,035 (485-8,972) | 229 (142-512) |
| 4BL3GAT | 88 (64-119) | 64 (39-94) | 122 (75-189) | 180 (117-317) |
| 4BL3GAV | 52 (32-74) | 44 (20-68) | 114 (83-150) | 70 (34-129) |
| 4BL3PAA | 197 (136-328) | 144 (75-277) | 4,000 (1,948-14,838) | 481 (44-988) |
| 4BL3AAA | 23 (17-30) | 82 (50-126) | >20,000e | 630 (306-11,328) |
a
Two-day-old larvae of A. aegypti, C. quinquefasciatus, and C. pipiens and 3-day-old larvae of A. quadrimaculatus were used for bioassays. Mortality was recorded after 24 h of exposure to a serial dilution of the toxins. The 95% confidence limits are indicated in parentheses. Bioassays for the Cry4B constructs used purified inclusion crystal protein produced in E. coli. ND, not determined. LC50, 50% lethal, concentration.
b
Data from reference 14.
c
Data from reference 2.
d
8% mortality was observed at this dose.
e
No mortality was observed.
f
17% mortality was observed at this dose.
g
8% mortality was observed at this dose.