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. 2003 Sep;69(9):5343–5353. doi: 10.1128/AEM.69.9.5343-5353.2003

TABLE 3.

Nonlinear regression of the binding curves to a Hill equationa

Toxin Bmax (fmol/μg) K′D (nM) Hill coefficient
4BRA 13.1 ± 0.5 6.9 ± 2.7 4.8 ± 0.8
4BL3PAT 9.3 ± 0.4 0.9 ± 0.2 2.8 ± 0.5
4BL3PAT* 18.3 ± 1.6 2.6 ± 0.5 2.3 ± 0.4
a

Each binding condition was conducted with 250 M excess homologous toxin added to reduce nonspecific binding, with the exception that 4BL3PAT* was conducted with 250 M excess 4BRA.