TABLE 1.
Effect of flavin contents in native- and deflavoenzyme preparations on the CL-20 biotransformation activities of various enzyme fractions from Pseudomonas sp. strain FA1 under anaerobic conditionsa
| Enzyme(s) | Total flavin content in native enzyme(s) (nmol/mg of protein) | CL-20 biotransformation activity of native enzyme(s) (nmol h−1 mg of protein−1) | Total Flavin content in deflavoenzyme(s) (nmol/mg of protein) | CL-20 biotransformation activity of deflavoenzyme(s) (nmol h−1 mg of protein−1) |
|---|---|---|---|---|
| Cell crude extract | 22.6 ± 1.3 | 15.6 ± 0.7 | ND | ND |
| Cytosolic soluble enzyme(s) | 5.5 ± 0.2 | 2.3 ± 0.05 | 1.2 ± 0.2 | 0.5 ± 0.05 |
| Membrane-associated enzyme(s) | 12.6 ± 0.6 | 11.5 ± 0.4 | 3.8 ± 0.3 | 2.7 ± 0.1 |
a
Data are means ± standard errors from triplicate experiments. ND, not determined.