TABLE 2.
Stoichiometries of reactants and products during biotransformation of CL-20a
| Reactant or product | Amt (nmol) | Molar ratio of reactant to product per reacted CL-20 molecule |
|---|---|---|
| Reactants | ||
| CL-20 | 20 | 1.0 |
| NADH | 90 | 4.5 |
| Products | ||
| Nitrite (NO2−) | 46 | 2.3 |
| Formate (HCOOH) | 34 | 1.7 |
| Nitrous oxide (N2O) | 29 | 1.5 |
a
CL-20 was biotransformed by a membrane-associated enzyme(s) (1 mg/ml) from Pseudomonas sp. strain FA1 at pH 7.0 and 30°C for 2.5 h under anaerobic conditions. The data are means of results of triplicate experiments.