TABLE 1.
Fluorescent and fluor-conjugated probes used in this study
Probe-fluor conjugatea | Supplier | Excitation/emission (nm) | Target |
---|---|---|---|
Syto9 | Molecular Probes | 488/522 | Nucleic acids |
SyproOrange | Molecular Probes | 470/570 | Protein |
Nile Red | Molecular Probes | 552/636 | Lipids, hydrophobic sites |
Arachis hypogaea-CY5 | Sigma | 650/670 | β-Gal(1-3)galNAc |
Canavalia ensiformis-TRITC | Sigma | 554/576 | α-d-Mannose |
α-d-Glucose | |||
Wisteria floribunda-FITC | Sigma | 494/520 | N-Acetyl-d-galactosamine |
Tetragonolobus purpureas-TRITC | Sigma | 554/576 | α-l-Fucose |
Tetragonolobus purpureas-CY5 | Sigma | 650/670 | α-l-Fucose |
Ulex europaeus-FITC | Sigma | 494/520 | α-l-Fucose |
Ulex europaeus-CY5 | Sigma | 650/670 | α-l-Fucose |
Ulex europaeus-Colloidal Gold | Sigma | Opaque | α-l-Fucose |
Datura stramonium-CY5 | Sigma | 650/670 | N-Acetylglucosamine |
N-Acetyllactosamine | |||
Sambucus nigra-CY5 | Sigma | 650/670 | α-NeuNAc(2-6)gal/galNAc |
Conjugated dye abbreviations: FITC, fluorescein isothiocyanate; TRITC, tetramethylrhodamine isothiocyanate; CY5, pentamethincyanine.