Figure 4.
Secretion of human Epo by C2C12 myotubes transduced with AAV-Epo. Confluent C2C12 myoblasts were differentiated into myotubes and transduced with 3 × 108 (□), 3 × 109 (▨), or 3 × 1010 (▪) vector particles of AAV-Epo. Twenty-four hours following a complete change of media, the secretion of Epo was measured (3, 8, and 14 days after transduction). AAV-lacZ-transduced myotubes secreted <2.5 milliunits/ml Epo. The bar graph depicts mean concentration of Epo per well per 24 hr determined in triplicate cultures ± SEM.