TABLE 2.
Isolates affiliated with the P. necessarius cluster and partial or nearly complete environmental 16S rRNA gene sequences with highest similarities
| Isolate | Accession no. | Source | Closest relativea | Accession no. | Sourceb | Similarity (%) | Reference |
|---|---|---|---|---|---|---|---|
| MWH-MoNR1 | AJ550649 | Lake Mondsee | Clone SC17 (p) | AY187480 | Mula-Mutha River, India | 99.8c | Satoor et al., unpublished |
| MWH-MoNR2 | AJ550650 | Lake Mondsee | Clone CRE-FL11 (p) | AF141441 | Columbia River, United States | 100.0 | 4 |
| MWH-MoIso1 | AJ550671 | Lake Mondsee | Clone FukuN66 (p) | AJ290078 | Fuchskuhle, Germany | 99.3 | 7 |
| MWH-MoIso2 | AJ550672 | Lake Mondsee | Clone CRE-FL11 (p) | AF141441 | Columbia River, United States | 100.0 | 4 |
| MWH-MoK1 | AJ550652 | Lake Mondsee | Clone SC17 (p) | AY187480 | Mula-Mutha River, India | 99.8c | Satoor et al., unpublished |
| MWH-MoK7 | AJ550673 | Lake Mondsee | Clone 06 | AF361192 | Rimov Reservoir, Czech Republic | 99.6 | 25 |
| MWH-MoK3 | AJ550653 | Lake Mondsee | Clone SC17 (p) | AY187480 | Mula-Mutha River, India | 99.8c | Satoor et al., unpublished |
| MWH-MoK4 | AJ550654 | Lake Mondsee | Clone 06 | AF361192 | Rimov Reservoir, Czech Republic | 99.6 | 25 |
| MWH-CaK1 | AJ550667 | Canal Roanne à Digoin | DGGE band wj 16 (p) | AF530937 | Walchensee, Germany | 98.8 | A. Bruns et al., unpublished |
| MWH-CaK5 | AJ550655 | Canal Roanne à Digoin | Clone CR-FL13 (p) | AF141398 | Columbia River, United States | 99.8 | 4 |
| MWH-VicM1 | AJ550651 | Lake Victoria | Clone CR99-2-58 (p) | AF429244 | Yangtze River, P. R. China | 100.0 | 22 |
| MWH-TaW3 | AJ550661 | Lake Tai hu | Clone CR99-2-49 (p) | AF429235 | Yangtze River, P.R. China | 99.8 | 22 |
| MWH-BeK4 | AJ550656 | Pond in Beijing | Clone CR-FL13 (p) | AF141398 | Columbia River, United States | 99.8 | 4 |
| MWH-JaW7 | AJ550658 | Yangtze River | Clone CR-FL13 (p) | AF141398 | Columbia River, United States | 99.8 | 4 |
| MWH-JaW21 | AJ550660 | Yangtze River | Clone CR99-2-49 (p) | AF429235 | Yangtze River, P.R. China | 99.8 | 22 |
| MWH-JaW9 | AJ550659 | Yangtze River | Clone CR99-2-58 (p) | AF429244 | Yangtze River, P.R. China | 100.0 | 22 |
| MWH-JaK3 | AJ550657 | Yangtze River | Clone FukuN33 | AJ289997 | Fuchskuhle, Germany | 99.8 | 7 |
| MWH-T1W13 | AJ550662 | Humble Park pond 1 | Clone CR-FL13 (p) | AF141398 | Columbia River, United States | 99.8 | 4 |
| MWH-T1W11 | AJ550670 | Humble Park pond 1 | Clone FukuN66 (p) | AJ290078 | Fuchskuhle, Germany | 99.3 | 7 |
| MWH-T1W2 | AJ550669 | Humble Park pond 1 | Clone CR99-7-39 (p) | AF429073 | Yangtze River, P.R. China | 99.8 | 22 |
| MWH-T2W13 | AJ550663 | Humble Park pond 2 | Clone CR99-7-39 (p) | AF429073 | Yangtze River, P.R. China | 99.8 | 22 |
| MWH-T2W3 | AJ550668 | Humble Park pond 2 | DGGE band WL8-0 (p) | AF497887 | Weser River, Germany | 100.0 | 23 |
| MWH-T2W17 | AJ550664 | Humble Park pond 2 | Clone FukuN66 (p) | AJ290078 | Fuchskuhle, Germany | 99.3 | 7 |
| MWH-HuK1 | AJ550665 | Tiger Hill pond | Clone ACK-C4 (p) | U85124 | Carry Pond, United States | 98.9 | 14 |
| MWH-HuW1 | AJ550666 | Tiger Hill pond | Clone FukuN66 (p) | AJ290078 | Fuchskuhle, Germany | 99.3 | 7 |
a
P, partial sequence; DGGE, denaturing gradient gel electrophoresis.
b
P.R. China, People's Republic of China.
c
Sequence seems to contain a 36-nucleotide-long sequence stretch from the vector at the 5′ end. This part of the sequence was omitted from the analysis.