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TLC analysis of C12-3-oxo-AHL produced by parent strain P. putida WCS358 (lane 1), by lon mutant derivative P. putida IBE4 (lane 2) and lon mutant derivative carrying a plasmid expressing Lon P. putida IBE4 (pBBRlon) (lane 3). Lane 4 contains the standards C10-3-oxo-AHL and C12-3-oxo-AHL. The AHLs were visualized with AHL-sensor strain E. coli (pSB1075); a volume corresponding to 5 × 108 cfu was loaded on the TLC assay.
