Table 1.
Pseudomonas strains, plasmids and oligonucleotides used.
| STRAINS | ||
| P. putida WCS358 | Pseudomonas putida, wt | [33] |
| P. putida IBE4 | lon1754::Tn5 of WCS358, Kmr | This study |
| P. aeruginosa 18577 | PA1803lon437::ISlacz/hah of PAO1, Tcr | [34] |
| PLASMIDS | ||
| pMOSBlue | Cloning vector, Ampr | Amersham-Pharmacia |
| pBluescriptKS | Cloning vector, Ampr | Strategene |
| pRK2013 | Kmr Tra+ Mob+, ColE1 replicon | [28] |
| pET28b | Expression vector, kmr | Novagen |
| pBBRmcs5 | Broad-host-range vector, Gmr | [35] |
| pSCHIA5 | pBluescriptKS carrying a 4.5 Kb BamHI/StuI fragment from IBE4 harboring some WCS358 and a fragment of Tn5, Ampr Kmr | This study |
| pB1A | pBluescriptKS carrying a 4.5 Kb EcoRI fragment from WCS358, Ampr | This study |
| pBBRlon | pBBRmcs5 carrying a 4.5 Kb EcoRI fragment from WCS358, Genr | This study |
| pPPUR3586H | pET28b derivative carrying ppuR of P. putida WCS358 | This study |
| pBBRppuR | pBBRmcs5 carrying the ppuR gene amplified from pPuR3586H with primers P0FW and P0RV | This study |
| pPPUI220 | pMP220 promoter probe vector carrying the ppuI promoter region, Tcr | [14] |
| pRSA220 | pMP220 promoter probe vector carrying the rsaL promoter region, Tcr | [14] |
| OLIGONUCLEOTIDES | ||
| P0FW | 5'-GGGTACCAATAATTTTGTTTAACTTTA-3' | [12] |
| P0RV | 5'-GGGATCCATTGCTCAGCGGTGGCAGC-3' | [12] |
| FW119 | 5'-CATGCCATGGCCCTACTGGTAATGG-3' | This study |
| RV122 | 5'-CCCAAGCTTGGGCGTGATCGATTTTTGC-3' | This study |
| Tn5ext | 5'-GAACGTTACCATGTTAGGAGGTC | [36] |