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. Author manuscript; available in PMC: 2008 Jul 1.
Published in final edited form as: Brain Behav Immun. 2006 Oct 31;21(5):569–580. doi: 10.1016/j.bbi.2006.09.001

Figure 3.

Figure 3

Zymosan’s effects and clonidine reversal of zymosan’s effects in apoptotic cells, lymphocytes, TGF-β1 (TGF) and anti-inflammatory macrophages in sciatic nerve 3 days after IFA plus saline (v+s), zymosan plus saline (z+s) or clonidine (z+c). A. Quantification of caspase- 3 (Casp3), ED1 and CD2 immunostaining. *P<0.05 comparison vs. IFA plus saline group; +P<0.05 comparison vs. zymosan plus saline group. B. Quantification of IL-10 expression. *P<0.05 comparison vs. IFA plus saline group. C. Quantification of apoptotic cells analyzed as proportion of co-expression of CD2 or ED1 in the caspase-3 set. +P<0.05 comparison vs. zymosan plus saline group. D. Quantification of apoptotic lymphocytes analyzed as number of CD2 and caspase-3 co-localization immunostaining and proportion of caspase-3 co-expression in the CD2 set. *P<0.05 comparison vs. IFA plus saline group; +P<0.05 comparison vs. zymosan plus saline group. E. Quantification of apoptotic macrophages analyzed as number of ED1 and caspase-3 co-localization immunostaining and proportion of caspase-3 co-expression in the ED1 set. *P<0.05 comparison vs. IFA plus saline group; +P<0.05 comparison vs. zymosan plus saline group. F. Quantification of TGF-β1 expression. *P<0.05 comparison vs. IFA plus saline group ; +P<0.05 comparison vs. zymosan plus saline group. G. Quantification of apoptotic macrophages analyzed as number of ED1 and caspase-3 co-localization immunostaining and proportion of caspase-3 co-expression in the ED1 set. *P<0.05 comparison vs. IFA plus saline group. H. Quantification of anti-inflammatory macrophages analyzed as number of ED1 and TGF-β1 co-localization immunostaining and proportion of co-expression of TGF-β1 in the ED1 set. *P<0.05 comparison vs. IFA plus saline group; +P<0.05 comparison vs. zymosan plus saline group.