Figure 4.
Effects of various chemicals on ATP-dependent genistein transport. Plasma membrane vesicles were incubated with 50 μm genistein, where vanadate (1 mm), cyclosporine A (5 μm), verapamil (5 μm), glybenclamide (150 μm), glutathione (1 mm), bafilomycin A1 (0.1 μm), gramicidin D (5 μm), nigericin (2 μm), valinomycin (2 μm), or NH4Cl (5 mm) were added prior to the addition of MgATP. Genistein transport was determined after 10 min of incubation. Data presented are means ± sd of three replicates. Asterisks indicate a statistically significant difference compared to +ATP control (P < 0.01).