Figure 5.

Effect of salt stress on growth of wild-type and ena1 plants. Protoplasts from wild type and ena1 were generated from 1-week-old protonemata to obtain identical starting material for the growth experiment. Protoplasts were allowed to recover for 2 weeks and then transferred to either standard medium (A) or medium with 100 (B) or 200 (C) mm NaCl. Growth of wild-type (black square) and ena1 plants (white square) was followed by measuring the diameter of 40 to 60 colonies from each treatment. Representative wild-type and ena1-15 colonies after 4 weeks of growth on standard medium (D) or medium with 100 mm NaCl (E) or after 8 weeks of growth on 200 mm NaCl (F). Bar = 1 cm.