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Micrographs of 2-week-old biofilms. (A, C, and E) Total bacteria were stained with CPI. (B, D, and F) Same fields; bacteria were detected after hybridization with a rhodamine-labeled 16S rRNA-targeted universal oligonucleotide probe (B), a probe specific for members of the δ proteobacteria (biofilm 1) (D), or a Legionella-specific probe (biofilm 10) (F). Bar, 5 μm.
