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. 2003 Sep;69(9):5463–5471. doi: 10.1128/AEM.69.9.5463-5471.2003

TABLE 2.

Biofilm conditions under which the presence of E. coli and other populations were observeda

Biofilm designation Disinfectant concn and exposure time No. of E. coli CFU inoculated
1 None 0
2 None 0
3 None 0
4 1 CT ClOH (1 ppm for 67 min) 0
5 0.2 ppm of residual ClOH starting on day 6 0
6 0.2 ppm of residual ClOH 0
7 None 106
8 1 CT ClOH 106
9 1 CT ClOH 106
10 4 ppm of NH2Cl for 155 min 106
11 2 ppm of residual NH2Cl 106
12 4 ppm of residual NH2Cl 106
a

Disinfectant type, concentration, and exposure time are summarized for 12 biofilms that were cryoembedded and sectioned for whole-cell hybridizations. E. coli 01571 was inoculated into biofilms 7 to 12.