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. 2003 Sep;69(9):5463–5471. doi: 10.1128/AEM.69.9.5463-5471.2003

TABLE 3.

E. coli detected in outflow from two flow chamber biofilm experiments by using Colilert Quanti-Tray MPNa

Biofilm designation Outflow sample day Time (days) after final bacterial inoculation Biofilm conditions No. of E. coli or coliform cells/100 ml of outflow
3 6 1 Control (no E. coli added) <1
3 7 2 Control <1
3 8 3 Control 3
3 9 4 Control 1
3 10 5 Control <1
8 11 6 1 CT ClOH (on day 14) >201b
11 2 ppm NH2Cl residual <1
12 4 ppm NH2Cl residual <1
7 12 7 Control 254
8 1 CT ClOH (on day 14) 4,425
7 14 9c Control 750
8 1 CT ClOH (on day 14) 504
10 4 ppm NH2Cl for 155 min (on day 14) 4,725
a

Bacteria were inoculated once a day during the first 5 days of the 2-week incubation period.

b

The E. coli concentrations in the sample exceeded the upper detection limit of the Quanti-Tray. Subsequent samples were diluted before incubation with the Colilert medium.

c

Samples collected after chlorine and monochloramine exposures.