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. 2007 Aug;144(4):1986–1999. doi: 10.1104/pp.107.098970

Figure 3.

Figure 3.

Analysis of the sinapate esters in the sng1, SALK_133207, and transformed sng1-6 mutants. Extracts were prepared from 7-d-old, etiolated dark-grown seedlings and analyzed by HPLC with UV detection at 335 nm. A, Extracts from Columbia wild-type, sng1-1, sng1-5, and sng1-6 mutants as well as the sng1-6 mutant transformed with the At2g23010 genomic construct (pCC579). B, Extracts from the T-DNA insertional mutant of At2g23010 (SALK_133207), and sng1-6 transformed with either the At2g22980 genomic construct (pCC881) or the At2g22990/SMT construct (pCC398). The sng1 mutants are defective in SMT and accumulate sinapoyl-Glc (SG) in their leaves instead of accumulating the sinapoylmalate (SM) that is found in wild-type plants. Compounds 1 (a disinapoylated monosaccharide) and 1,2-disinapoyl-Glc do not accumulate in the sng1-6 mutant, both compounds accumulate in the sng1-6/pCC579 mutant, but only 1,2-disinapoyl-Glc accumulates in SALK_133207, sng1-6/pCC881, and sng1-6/pCC398.