Evolutionary relationships of 2C internal endoduplications and of shaft sequences. A, Substitution patterns of aligned internal repeats in the four 2C subdomains of Sag1 and Sad1. Shading and symbols as in Figure 2. B, Tajima-Nei distances of alignable shaft sequences, where top scale is 2× the bottom scale. Top (I-bars), Between-species distances between the two cell wall sequences, Gp1 and Vsp3, and between Homology I and II regions of the Sag1 and Sad1 agglutinins. Bottom, Within-species endoduplicated repeats in the four 2C subdomains displayed as distance trees (neighbor-joining method).