Figure 2.

Native-PAGE/activity staining of developing endosperm in rice SSIIIa mutant lines and the wild type. The numbers above the lanes are the volumes (μL) of the crude enzyme extract applied onto each lane. A, Native-PAGE/SS activity staining. The SSIIIa and SSI activity bands are indicated by arrowheads. B, Native-PAGE/DBE activity staining. The ISA (isoamylase), PUL (pullulanase), and PHO (phosphorylase) activity bands are indicated by arrowheads. C, Native-PAGE/BE activity staining. The BEI, BEIIa, and BEIIb activity bands are indicated by arrowheads. ‘Nip’, Wild type of ss3a-1, ss3a-1, SSIIIa mutant by Tos17 insertion; ‘T65’, Wild type parent of ss3a-2, ss3a-2, SSIIIa mutant induced by chemical mutagenesis. [See online article for color version of this figure.]