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. 2007 Aug;144(4):2009–2023. doi: 10.1104/pp.107.102533

Figure 6.

Figure 6.

Size separation of endosperm starch and purified amylopectin from the SSIIIa mutant, ss3a-1, and the wild type, ‘Nip’. A and B, Elution profiles by gel filtration chromatography through Sephacryl S-1000SF of starch (A) and purified amylopectin (B) from ‘Nip’ (black lines) and ss3a-1 (gray lines). C and D, Elution profiles of isoamylase-debranched starch (black lines) and purified amylopectin (gray lines) by gel filtration chromatography through Toyopearl HW55S-HW50S columns from ‘Nip’ (C) and ss3a-1 (D). Each fraction was divided according to the following range of λmax values of the glucan-iodine complex: Fr. I, λmax ≥ 620 nm; Fr. II, 540 nm ≤ λmax < 620 nm; Fr. III, λmax < 540 nm. E, Percentage comparisons of each fraction separated by gel filtration (C and D) in the total carbohydrate of endosperm starch and purified amylopectin from ‘Nip’ and ss3a-1.