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Immunohistochemical detection of AGT in ΔMGMT-transduced K562 cells. K562 cells (1 × 105) were infected with dilutions of amphotropic supernatant collected from Am12ΔMGMT clone 20. At a 1:50 dilution of supernatant, ≈10% of the cells expressed nuclear human AGT when reacted with monoclonal antibody mT3.1.
