Figure 5.

Clonogenic survival of transduced CD34 cells following treatment with BG plus BCNU. CD34 cells were transduced by coculture method (A) or retroviral supernatant in the presence of allogenic stroma (B). Transduced cells were treated with 10 μM BG and 0–40 μM BCNU and plated in methylcellulose in triplicate, and colonies were enumerated in 7–10 days. Data points represent the mean ± SEM of five experiments (A) and three experiments (B) from separate donors. P < 0.001 for the comparison ΔMGMT vs. lacZ.