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Expression of the Shaker family channel genes of rice in various tissues. Total RNA was isolated from leaves and roots of rice seedlings and flag leaves, internodes, mature leaves, leaf sheaths, caryopses, and rachides of mature plants. Transcripts were detected by RT-PCR. Rice actin was amplified as a loading control. The PCR products were separated in 0.8% (w/v) agarose gels and stained with ethidium bromide.
