Figure 3.

Detection of PpcA and PK mRNA by RT-PCR. RNA was extracted from wild-type (Wt) leaves collected at midnight (D) and midday (L) and from leaves of progeny of A47b#6 and R111a#3 collected at midday. Plants were growing in a naturally lit glasshouse during January and February, 2007. RNA was retrotranscribed and samples of the first-strand synthesis were used in PCR reactions with gene-specific primers as described in “Materials and Methods.” Similar results were obtained in two independent experiments.