Table II.
Leaf metabolite content and net CO2 assimilation rates
Four different progeny were measured for each transgenic line and wild type. Gas-exchange measurements were made at external CO2 of 350 μbar, 2,000 μmol quanta m−2 s−1, and a leaf temperature of 28°C before rapidly freeze clamping the leaf sections. Statistical analysis was conducted using ANOVA and there were no significant differences between genotypes.
| CO2 Assimilation and Metabolites | Wild Type | A47b#6 | R111a#3 |
|---|---|---|---|
| CO2 assimilation rate (μmol m−2 s−1) | 55.0 ± 2.5 | 52.5 ± 2.3 | 50.8 ± 3.1 |
| Malate (μmol m−2) | 2,611 ± 257 | 2,605 ± 434 | 1,820 ± 150 |
| Asp (μmol m−2) | 237 ± 39 | 279 ± 45 | 195 ± 19 |
| Pyruvate (μmol m−2) | 308 ± 39 | 268 ± 15 | 241 ± 36 |
| PEP (μmol m−2) | 88 ± 4 | 97 ± 6 | 82 ± 7 |
| Triose-P (μmol m−2) | 261 ± 57 | 251 ± 32 | 273 ± 30 |
| Phosphoglyceradehyde (μmol m−2) | 365 ± 15 | 361 ± 23 | 365 ± 48 |
| Ribulose bisphosphate (μmol m−2) | 89 ± 16 | 68 ± 16 | 52 ± 39 |