Table 1.

Mean LD₅₀ values for neo-control and HA-BAX SW626 transfectants

Drug	LD50,* μM		P value†	LD50(neo)/ LD50(BAX)
	neo-control	HA-BAX
Paclitaxel	0.20 ± 0.03	0.02 ± 0.01	0.001	10.0
Vincristine	0.142 ± 0.11	0.006 ± 0.001	0.0001	23.7
Doxorubicin	0.21 ± 0.07	0.035 ± 0.02	0.001	6.0
CBDCA	9.5 ± 2.0	12.0 ± 3.0	0.2	0.79
VP-16	2.8 ± 0.8	2.6 ± 0.9	0.2	1.08
Hydroxyurea	0.025 ± 0.005	0.026 ± 0.009	0.2	0.96

^*Cells were grown at subconfluent density in plastic wells over a 3-day period in the presence of various drugs as indicated, followed by trypsinization and assessment of viability by trypan blue exclusion. LD₅₀ values reflect the amount of drug necessary to achieve 50% cell kill compared to cells grown in medium alone. Data are expressed as mean ± SEM LD₅₀ for either neo-control clones (n = 3, clones B10, A10, and F8) or HA-BAX clones (n = 3, clones D8, A9, and D7), as indicated. 

^†P values determined by two-sided Student’s t test. Significant enhancement of cytotoxicity was observed in HA-BAX-expressing clones treated with paclitaxel, vincristine, and doxorubicin.