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. Author manuscript; available in PMC: 2007 Sep 17.
Published in final edited form as: Lung Cancer. 2007 Jan 17;56(2):167–174. doi: 10.1016/j.lungcan.2006.12.003

Fig. 2. Regulation of RANTES and IL-8 promoter activity after site-directed mutagenesis.

Fig. 2

(A) A549 cells were transfected with intact or point mutated RANTES promoter-luciferase gene construct as indicated, and CMVβ-gal. These were treated for 4 h with TNF-α (10 ng/ml) or TPA (100 ng/ml) or the combination of both compounds, and lysed to determine relative luciferase activities after normalization for β-galactosidase activity. Data (n = 6) are shown as fold inductions over basal activity and are plotted as means ± SE. (B) A549 cells were transfected with intact or mutated IL-8 promoter-luciferase gene construct as indicated, and CMVβ-gal. These were then treated as in (A). Data (n = 6) are shown as fold inductions over basal activity and are plotted as means ± SE.