TABLE 1. Quantitative analysis of the protection of lysine residues of β protein from biotinylation upon DNA complex formation.

The values in the table indicate the ratio of intensities for each lysine-containing peptide before and after biotinylation of the free β protein (β/(β + biotin)) or the β protein-DNA complex ((β + DNA)/(β + DNA + biotin)). Each value in the table is derived from integration of peaks in the extraction chromatograph and is the average of five separate experiments. A high value indicates that the corresponding lysine residue is biotinylated.

Fragments	Modified K	β/(β+biotin)	(β+DNA)/(β+ DNA + biotin)	Protection
1–11	11	21.4 ± 4.2	17.4 ± 3.9	−
37–61	36, 61	11.4 ± 3.1	1.1 ± 0.1	+
62–69	69	5.0 ± 2.5	1.2 ± 0.3	+
138–148	148	2.0 ± 0.8	1.3 ± 0.3	+
155–161	154	12.4 ± 2.9	1.2 ± 0.2	+
162–172	172	21.4 ± 3.8	1.3 ± 0.3	+
215–229	214	5.4 ± 1.7	5.3 ± 2.8	−
234–245	245	96.0 ± 20.5	68.3 ± 9.9	−
246–251	251	67.6 ± 15.3	103.3 ± 30.4	−