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. Author manuscript; available in PMC: 2008 Apr 1.

Published in final edited form as: Mol Immunol. 2007 Feb 26;44(11):2884–2892. doi: 10.1016/j.molimm.2007.01.014

Fig. 5 — Spleens, mesenteric, pancreatic, and peripheral lymph nodes from individual recipients were harvested, processed, counted, and phenotyped by flow cytometric analysis to determine effective CD4⁺CD25⁺ T cell expansion and repopulation. Each cellular compartment was phenotyped using the surface markers specific for CD4⁺CD25⁺ T cells, CD3-FITC, CD25-PE, and CD4-APC and statistically analyzed by Mann-Whitney U test. (A) Absolute numbers of CD4⁺CD25⁺ T cells in individual recipients were calculated for each cellular compartment based on percent of CD3⁺CD4⁺CD25⁺ cells recovered. Absolute numbers of CD4⁺CD25⁺ cells calculated for each compartment were added to determine total cell recovery. Absolute numbers of (B) splenic and (C) pancreatic lymph node CD4⁺CD25⁺ T cells in individual recipients were calculated based on percent of CD3⁺ CD4⁺CD25⁺ cells recovered in spleen and pancreatic lymph nodes, respectively. * P<0.01. †P<0.05.

Fig. 5 — Spleens, mesenteric, pancreatic, and peripheral lymph nodes from individual recipients were harvested, processed, counted, and phenotyped by flow cytometric analysis to determine effective CD4⁺CD25⁺ T cell expansion and repopulation. Each cellular compartment was phenotyped using the surface markers specific for CD4⁺CD25⁺ T cells, CD3-FITC, CD25-PE, and CD4-APC and statistically analyzed by Mann-Whitney U test. (A) Absolute numbers of CD4⁺CD25⁺ T cells in individual recipients were calculated for each cellular compartment based on percent of CD3⁺CD4⁺CD25⁺ cells recovered. Absolute numbers of CD4⁺CD25⁺ cells calculated for each compartment were added to determine total cell recovery. Absolute numbers of (B) splenic and (C) pancreatic lymph node CD4⁺CD25⁺ T cells in individual recipients were calculated based on percent of CD3⁺ CD4⁺CD25⁺ cells recovered in spleen and pancreatic lymph nodes, respectively. * P<0.01. †P<0.05.