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. Author manuscript; available in PMC: 2008 Apr 1.
Published in final edited form as: Mol Immunol. 2007 Feb 26;44(11):2884–2892. doi: 10.1016/j.molimm.2007.01.014

Fig. 6. Analysis of FoxP3 mRNA expression and suppressive activity of CD4+CD25+ T cells in lymph node of long-term SA-FasL survivors.

Fig. 6

Fig. 6

(A) Total RNA was extracted from whole pancreatic lymph node. cDNA was amplified in duplicate by real-time PCR for GAPDH and FoxP3. FoxP3 mRNA levels were normalized relative to GAPDH mRNA expression and statistically analyzed by Kruskal-Wallace test. Data are presented as the fold-change relative to control, sorted CD4+CD25- T cells. P>0.05. (B) CD4+CD25+ cells sorted from healthy naïve NOD peripheral lymph node (control) and long-term SA-FasL survivor pooled peripheral and pancreatic lymph nodes were cultured with control CD4+CD25- T cells at different ratios and evaluated for suppressive function. Data presented is representative of three suppression assays performed evaluating suppressive activity of CD4+CD25+ from long-term SA-FasL survivors.