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. Author manuscript; available in PMC: 2008 Jul 5.
Published in final edited form as: Virology. 2007 Mar 1;363(2):319–332. doi: 10.1016/j.virol.2007.02.005

Fig. 2. Initial marker rescue mapping of DL1-3.

Fig. 2

A) Primer pairs used to construct PCR products for marker rescue. Products amplified using a library of primers are shown as bars above the region they amplify on a HindIII restriction map of the vaccinia virus genome. Each product is approximately 5 kb in length. The PCR product numbers shown here are used throughout our study. B) Confluent dishes of BSC40 cells were infected with the Dts38 helper virus and co-transfected with wild-type genomic DNA and the indicated PCR product. After a 4 day incubation at 37°C in the presence of IBT, dishes were stained with crystal violet.