Table 1.
Primary antibodies used for immunoblot analysis.
| MUCIN | ANTIBODY | BLOCKING SOLUTION | EPITOPE | SOURCE/REFERENCE |
|---|---|---|---|---|
| MUC1 | HMFG-1 | 5% BLOTTO* | TR† (APDTR), glycosylation sensitive | Biodesign, Saco ME (Taylor-Papadimitriou et al., 1981; Burchell J and Taylor-Papadimitriou, 1993; Price et al., 1998) |
| MUC1 | HMFG-2 | 5% BLOTTO | TR (DTR), glycosylation sensitive | Biodesign, Saco ME (Taylor-Papadimitriou et al., 1981; Burchell J and Taylor-Papadimitriou, 1993) |
| MUC1 | 214D4 | 5% BLOTTO in PBS | TR (PDTR), glycosylation independent | J. Hilkens, Netherlands Cancer Institute, Amsterdam, The Netherlands (Wesseling et al., 1995; Price et al., 1998) |
|
| ||||
| MUC2 | PMH1 | 5% BLOTTO in TBS | GalNAC-TR | U. Mandel, University of Copenhagen, Denmark (Reis et al., 1998) |
| MUC2 | PH1417 | 10% BLOTTO in TPBS | C-terminus(aa 4995–5013) | G. Hansson, Göteburg University, Sweden (Axelsson et al., 1998) |
| MUC2 | PH1491 | 10% BLOTTO in TPBS | N-terminus(aa 1167–1180) | G. Hansson, Göteburg University, Sweden (Asker et al., 1998) |
|
| ||||
| MUC4 | 1G8 | 3% BSA/0.5% TTBS | Ectodomain of ASGP-2 | Zymed, San Francisco, CA (Zhang et al., 2005) |
| MUC4 | 8G7 | 5% BLOTTO in PBS | TR | S.K. Batra, University of Nebraska Medical Center, Omaha, Nebraska(Moniaux et al., 2004) |
|
| ||||
| MUC5AC | 791 | 3% Fish Gel | D3 domain | I.K. Gipson (Argueso et al., 2002) |
| MUC5AC | CLH2 | 5% BLOTTO | TR | U. Mandel, University of Copenhagen, Denmark (Reis et al., 1997); Vector Labs, Burlingame, CA |
| MUC5AC | 1-13M1 | 5% BLOTTO | N-terminus: cys-2, cys-4 domains | NeoMarkers, Fremont, CA (Nollet et al., 2004b; Nollet et al., 2004a) |
|
| ||||
| MUC5B | 799 | 3% Fish Gel | D4 domain | I.K. Gipson (Gipson et al., 2001) |
| MUC5B | Eu-MUC5Ba | 5% BLOTTO in PBS | Cysteine-rich domain | D.M. Swallow, University College, London, UK (Rousseau et al., 2003) |
|
| ||||
| MUC7 | PANH3 | 5% BLOTTO | N-terminus(aa 72–92) | U. Mandel, University of Copenhagen, Denmark (Nielsen et al., 1996) |
| MUC7 | Eu-MUC7a | 5% BLOTTO in PBS | Histatin-like domain | D.M. Swallow, University College, London, UK (Rousseau et al., 2003) |
|
| ||||
| MUC16 | OC125 | 5% BLOTTO | TR | Dako Corporation, Carpenteria, CA (Bast et al., 1981) |
| MUC16 | M11 | 5% BLOTTO | TR (Different from OC125) | NeoMarkers, Fremont, CA (Nustad et al., 1996) |
| MUC16 | H185 | 5% BLOTTO in TBS | Carbohydrate epitope | I.K. Gipson (Watanabe et al., 1995; Argueso et al., 2003a) |
*
BLOTTO = Non-fat Dry Milk diluted (w/v) in TTBS, except as noted above.
†
TR = Tandem repeat